Recovery of R11, a Novel Bladder-specific Cell Permeable Peptide, in the Rabbit Bladder and Antibiotic Efficacy of R11-tagged to Ciprofloxacin in Vitro
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چکیده
Hypothesis / aims of study Prior studies in a mouse model demonstrated that a new cell permeable peptide, R11, is bladder specific and can be retrieved in the bladder wall after intra-vesical instillation. This unique tissue specificity makes R11 a suitable candidate for intra-vesical antibiotic delivery for recurrent or relapsing bacterial cystitis. The rabbit bladder model is commonly used for pre-clinical studies on bacterial cystitis because the bladder surface glycosaminoglycan layer (GAG) composition is very similar to humans (ref 1). Therefore, we tested the uptake efficiency of R11 administered intra-vesically in the rabbit model recovery and evaluated the efficacy of R11-conjugated to an established fluoroquinolone antibiotic (Ciprofloxacin) in vitro.
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